Synthetic Gene Circuits: Design with Directed Evolution
نویسندگان
چکیده
منابع مشابه
Synthetic gene circuits: design with directed evolution.
Synthetic circuits offer great promise for generating insights into nature's underlying design principles or forward engineering novel biotechnology applications. However, construction of these circuits is not straightforward. Synthetic circuits generally consist of components optimized to function in their natural context, not in the context of the synthetic circuit. Combining mathematical mod...
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De novo computational design of synthetic gene circuits that achieve well-defined target functions is a hard task. Existing, brute-force approaches run optimization algorithms on the structure and on the kinetic parameter values of the network. However, more direct rational methods for automatic circuit design are lacking. Focusing on digital synthetic gene circuits, we developed a methodology ...
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MOTIVATION In principle, novel genetic circuits can be engineered using standard parts with well-understood functionalities. However, no model based on the simple composition of these parts has become a standard, mainly because it is difficult to define signal exchanges between biological units as unambiguously as in electrical engineering. Corresponding concepts and computational tools for eas...
متن کاملSynthetic Gene Circuits
Barbara Jusiak1,2, Ramiz Daniel1,3, Fahim Farzadfard2–5, Lior Nissim1,3, Oliver Purcell2,3, Jacob Rubens1,3,5, and Timothy K. Lu1–6 1Massachusetts Institute of Technology, Research Laboratory of Electronics, Cambridge, MA 02139, USA 2Massachusetts Institute of Technology, Department of Biological Engineering, Cambridge, MA 02139, USA 3Massachusetts Institute of Technology, Synthetic Biology Cen...
متن کاملSynthetic biology: integrated gene circuits.
A major goal of synthetic biology is to develop a deeper understanding of biological design principles from the bottom up, by building circuits and studying their behavior in cells. Investigators initially sought to design circuits "from scratch" that functioned as independently as possible from the underlying cellular system. More recently, researchers have begun to develop a new generation of...
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ژورنال
عنوان ژورنال: Annual Review of Biophysics and Biomolecular Structure
سال: 2007
ISSN: 1056-8700,1545-4266
DOI: 10.1146/annurev.biophys.36.040306.132600